Example of a Microbiology Unknown Lab Report

 

examples of unknown lab reports for microbiology

UNKNOWN LAB REPORT. Unknown Number Tyler Wolfangel. April 29, BIO Introduction. The study of microbiology requires not only an academic understanding of the microscopic world but also a practical understanding of lab techniques and procedures used to identify, control, and manipulate microorganisms. Jun 26,  · Microbiology. UNKNOWN LAB REPORT. Kateryna Petrakova. INTRODUCTION. Microorganisms cause a great amount of diseases. For healthcare providers it is very important to be aware of what organisms are pathogenic and cause a disease and, . MICROBIOLOGY UNKNOWN LAB REPORT. Unknown Number Jennifer Mills. April 30, Microbiology, BIO Spring/ INTRODUCTION In the past, it has been vital to distinguish the identities of microorganisms in the world.


How to Write Microbiology Unknown Lab Report | Example Paper


It is essential in medicine to be able to identify different microorganisms for diagnosing the cause of various diseases, and determining the action for treatment. This study was done using the methods of sterile technique and biochemical tests that have been practiced throughout the semester in the microbiology laboratory.

The purpose of this study was to identify the two different bacterium from a mixed culture, one Gram positive and one Gram negative. The lab instructor gave out a test tube labeled examples of unknown lab reports for microbiology with two unknown bacterium.

The sterile techniques and methods were followed using the laboratory manual by McDonald et. The first procedure performed was applying a series of streaks onto a nutrient agar with a sterile inoculating loop using the quadrant streak method, and placing it in the incubator at 37 degrees Celsius to grow for two days.

The plate was studied and one distinct colony was identified, examples of unknown lab reports for microbiology. A Gram stain was performed on the isolated colony. The Gram stain procedure was carefully followed according to the steps in the laboratory manual. Gram positive purple cocci bacteria were identified using a microscope. The Gram negative were unable to be isolated from the nutrient agar.

In order to get the Gram negative to grow, another test was performed taking a sample from the original tube using the sterile inoculating loop, and streaking a MacConkey agar placing it in the incubator at 37 degrees Celsius. All plates were labeled, and tests noted in the journal. The Gram negative bacterium was still not growing on MacConkey agar so another inoculation was done using an EMB agar.

Still unable to get results examples of unknown lab reports for microbiology the EMB agar, the lab instructor gave an isolated tube labeled alternate A5. Table 1 and 2 list the tests, purpose, reagents, observations and results for each bacterium. All of the following tests were performed on these unknowns:. The unknown number was streaked on a nutrient agar plate, and a Gram stain was performed. The results were Gram positive cocci, examples of unknown lab reports for microbiology.

The Gram stain for Gram negative was taken from alternate A5 with the results of Gram negative rods. Table 1 and 2 lists all of the biochemical test, their purpose and results. The results are also shown in flow chart form. Pseudomonas aeruginosa. The results of the Gram positive bacterium after all biochemical tests were performed was identified as Enterococcus faecalis.

A Gram stain discovered that the bacteria were cocci shaped which narrowed the results down to three different bacteria. The first biochemical test performed was a negative Urea test which left two bacteria. The last test was a negative Catalase test that led to the only Gram negative bacteria left which was E. A Nitrate test was performed to confirm the result of E. This result was confirmed by Lab instructor. There were no problems encountered with finding this conclusion.

The results for the Gram negative bacterium after tests were performed was identified as Klebsiella pneumonia. There were problems in the beginning getting an isolated culture for the Gram negative bacterium.

Due to problems isolating a pure culture on the original nutrient agar, two different agar plates were inoculated using a sterile swab with the original broth, and spread onto the EMB and MacConkey agars.

This was to try and get the Gram negative bacterium to grow and inhibit the Gram positive, examples of unknown lab reports for microbiology. There was still no growth on both tests. Examples of unknown lab reports for microbiology Lab instructor gave an isolated Gram negative tube labeled alternate A5.

A Gram stain was performed and identified as Gram negative rods, examples of unknown lab reports for microbiology. The first biochemical test performed was a Simmons Citrate test with a negative result. The lab instructor said that the test result was wrong and to try another test.

This led to the Nitrate test with a positive result that narrowed it down to four bacteria. A SIMs test was performed to determine if bacteria can reduce sulfur which produces hydrogen sulfide to determine the ability of an organism to split indole from tryptophan, and to check for motility. The final test performed was a positive Urea test with the conclusion of K.

This result was confirmed by the Lab instructor. The bacterium K. Those who are most at risk are the elderly and sick patients within a clinical setting who are receiving treatment for other conditions such as breathing machines, intravenous catheters and those taking antibiotics for an extended period.

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How to Write a Microbiology Lab Report: 14 Steps (with Pictures)

 

examples of unknown lab reports for microbiology

 

Jul 18,  · UNKNOWN LAB REPORT. Unknown Number Penny Pitman. General Microbiology BIO Spring INTRODUCTION It is essential in medicine to be able to identify different microorganisms for diagnosing the cause of various diseases, and determining the action for treatment. Jan 16,  · Unknown streak A was red and had rods, therefore it was a gram negative. These tests were done and followed by the help of the McDonald lab manual. Tabel 1 lists the test, purpose, reagents and results of Unknown streak A. All of the following tests were performed on . Jun 26,  · Microbiology. UNKNOWN LAB REPORT. Kateryna Petrakova. INTRODUCTION. Microorganisms cause a great amount of diseases. For healthcare providers it is very important to be aware of what organisms are pathogenic and cause a disease and, .